Enhancing the in vitro release of total flavonoids extract from Dracocephalum moldavuca composite phospholipid liposomes optimized by response surface methodology

The present study was undertaken to optimize the preparation conditions of total flavonoids extract from Dracocephalum Moldavuca composite phospholipid liposome [TFDMCPL]by response surface methodology [RSM] and to investigate the in vitro release [IVR] of TFDMCPL. Method of ethanol injection was adopted to prepare TFDMCPL. The single factor experiments were used for the key experimental factors and their test range. Based on the single factor experiments, with encapsulation efficiency [EE] Size of TFDMCPL and polymey disperse index [PDI] as dependent variable, central composite design was adopted to optimize preparation technology by taking content of phospholipid and content of cholesterol as independent variables, fitting of various mathematical equations were performed using a statisitical software of Design-Expert 8.0.6. Preparation parameters were optimized through response surface plotted by optimum fitting equations, optimized procedure was validated through experimental preparation of TFDMCPL. Optimum preparation technology was as following: phospholipid 505mg and cholesterol 50mg. Under these condition, encapsulation efficiency was 90.2+/-1.2%, size of TFDMCPL was 115.6+/-4.3nm, PDI was 0.169+/-0.015 and Zeta potential was -15.38+/-0.5. These indicated that TFDMCPL with high entrapping efficiency and small particle size could be prepared by the ethanol injection method. And TFDMCPL were found to enhance the release of drugs more effectively than TFDM based on the in vitro model

Optimization of extraction process for compund kaliziran gel with orthogonal design / 中国中药杂志

<p><b>OBJECTIVE</b>To optimize the extraction process of compund kaliziran gel.</p><p><b>METHOD</b>Orthogonal design was used to optimize the extracting process of eight herb medicines with the extract yield, the content of psoralen, isopsoralen and osthole as indexes, and to optimize the extracting process of Polygonum multflorumi and Seman sinapis albae with the extract yield and the content of 2,3,5,4'-tetrehydroxy-stilbene glucoside as indexes.</p><p><b>RESULT</b>The optimum extraction progress was as follows:eight herb medicines were extract 2 times for 1 hour with 8 times of 60% alcohol; P. multflorumi and S. sinapis albae were extract 2 times for 1 hour with 8 times of 40% alcohol.</p><p><b>CONCLUSION</b>The optimum extraction processes are stable and feasible.</p>

Quality standard for Hanshi Tougu Tablet / 中成药

AIM: To establish the quality standard for Hanshi Tougu Tablets(Radix Aconiti Praeparata,Radix Aconiti Kusnezoffii Praeparata,Ramulus Cinnamomi,Rhizoma Atractylodis, Semen Sinapis,Radix Aucklandiae,Radix Glycyrrhizae). METHODS: Rhizoma Atractylodis,Semen Sinapis,Radix Glycyrrhizae in Hanshi Tougu Tablet were identified by TLC.The content of cinnamaldehyde and costunolide in Hanshi Tougu Tablets were determinded by HPLC. RESULTS: Rhizoma Atractylodis,Semen Sinapis,Radix Glycyrrhizae,Radix Aconiti Praeparata and Radix Aconiti Kusnezoffii Praeparata could be identified by TLC.Cinnamaldehyde showed a good linear relationship at the range of 2.104 ?g?mL~(-1)-52.60 ?g?mL~(-1),r=0.999 8(n=5), and the recovery was(99.62%,) RSD was(0.88%).Costunolide also showed a good linear relationship at a range of(0.036) mg?mL~(-1)-0.54 mg?mL~(-1),r=(0.999 9)(n=5),and the recovery was 96.10%,RSD was 0.15%. CONCLUSION: The method is simple,accurate and with strong specificity and can be used for the quality control of Hanshi Tougu Tablets.